How to compute correctly the heat flux in order to obtain a stable thermal/thermo-hydraulic coupled simulation ?

Dear all, I am trying to couple a simulation with two codes, say code A and code B (different discretizations). For code A we solve simply the conduction equation (solid), while in B we solve the...

24 November 2020 8,961 2 View

How do I run a western blot following co-ip?

This is the first time I do any sort of IP experiment and I was wondering if there is a certain procedure to follow for the western blot after the last elution step in the co-ip protocol. I am...

22 September 2020 3,688 3 View

How to properly normalize my absolutely quantified mRNA expression?

My PI wanted to use a standard curve to absolutely quantify mRNA expression via RT-qPCR. Her idea was to create the standard curve from a stimulated cell line that expressed the gene of interest...

19 September 2018 4,368 16 View

Can someone help with standard curve for competitive ELISA?

I used a competitive ELISA to look for collagen IV. I got the standard curve numbers. The instructions in the manual state that the data may be linearized by plotting the log of concentrations...

22 March 2018 6,633 2 View

Is there a specific method to set a Mach Zehnder interferometer for surface relief grating inscription on polymer thin films?

Hello, I have built a Mach-Zhender interferomer in order to irradiate thin films of polymer (PMMA) with an interference pattern. These polymer thin films are doped with metallic precursors, and...

25 May 2016 4,245 4 View

What initiator should I use to prepare a nanocomposite made of Cobalt nanoparticles dispersed in a PMMA matrix?

29 February 2016 6,294 1 View

Calculating the effective mass manually

I am trying to calculate the effective mass of electrons and holes manually from the band structure of a 2D system I am working on. I have only considered the maximum valence band and the minimum...

01 January 1970 4,055 3 View

How to compute correctly the heat flux in order to obtain a stable thermal/thermo-hydraulic coupled simulation ?

Dear all, I am trying to couple a simulation with two codes, say code A and code B (different discretizations). For code A we solve simply the conduction equation (solid), while in B we solve...

01 January 1970 9,673 3 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

What are the application of Fixed points thoery in Chemistry.?

It is said that fixed point theory has lot of applications not in the field of mathematics only but also in various areas. So I am looking for applications in Chemistry only..

26 February 2021 3,533 1 View

How to interpolate from a time-series database ?

Hi, I am having trouble with a problem, in the field of Optimal Control and the generation of optimal time-series. Let's consider a system, whose dynamics are represented by dx/dt =...

24 February 2021 9,105 2 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

Helix antenna coupling problem?

Dealing with a helix antenna, made with flexible material and not rigid like using a PCB board or wire. At the beginning of the helix is too close to the ground plane, causing coupling. How can I...

21 February 2021 4,453 3 View

How to calculate the Atomic Polar Tensor using DFT ?

Dear friends, I am a beginner at DFT calculation. I have done a structure optimization using DFT- B3LYP/6-31G*, Nevertheless, I don't know How to calculate the Atomic Polar Tensor (APT) in...

17 February 2021 9,082 3 View

Is there any effective FEM solver to perform simulation of balloon inflation with large deformation (e.g. 300% strain)?

Hi! Thank you for your time, attention, and support. I am currently using FEM solver Abaqus to perform a set of balloon inflation simulations, trying to understand the deformation shape, stress,...

14 February 2021 2,524 4 View