Hi
I am interested in measuring mitochondrial content by Flow cytometry, I search a bit and almost all mitochondrial dyes has been recommended, but there are very short description in papers in method nd discusion part. when I was doing confocal, I noticed MitoTracker Green FM and MitoTracker Red CMXRos results different than what has been described. I stained cells with both probes and I was expecting to have more green signal, as Green is supposed to stain all mitochondria, and CMXRos accumulates in membranes with higher potential. But surprisingly I had more red signal than green. I also have JC1 and TMERM to do flow cytomtry, but not sure which one shall I use. I would be very grateful if you could kindly share your experience/ideas.
kind regards
Somaieh
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