How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

GC-Headspace (HS-GC) chromatography for Forensic toxicological and Food expertises?

Dear Colleagues! How correct and appropriate is it to conduct forensic toxicological and food examination of volatile substances (ethanol, methanol, propanol, ethyl glycol, etc.) in blood and...

24 January 2021 5,645 2 View

How to determinate phosphatidylcholine content in cow milk?

I would like to determinate the phosphatidylcholine content in cow milk. We only have a Shimadzu Prominence HPLC (High Performance Liquid Chromatography) with a fluorescent detector for the...

18 January 2021 8,370 6 View

How to measure abundance in an LC-MS/MS experiment?

Is the chromatographic peak a valid measure of a compounds` (relative) abundance in LC-MS data? We have recorded three MS/MS spectra; their retention time falls into a specific chromatographic...

13 January 2021 6,685 6 View

What are state-of-the-art methods/techniques/algorithms for the root-cause analysis of the anomalies?

I have been looking for research articles that target the question "why did an anomaly occur?", however, most of the papers that I found are related to "if an anomaly occurred?". I searched Google...

12 January 2021 7,951 3 View

The chemical and microbiological properties of camel milk do have a significant effect on shelf life of camel milk powder compared to cow milk powder?

I need to know effect of heat treatments that are made when making camel milk powder on chemical , microbiological properties and shelf life of camel milk powder compared to cow milk powder. Thanks

11 January 2021 7,097 12 View

Where can I purchase NanoLC emitter fused silica picoTip?

Hi, I'm working with NanoLC/MS for both Metabolomics and Proteomics. We usually make our nano columns using emitters from NewObjective. Unfortunately New Objective, due to the pandemic, is now...

10 January 2021 1,176 4 View

How weight of carrier and coating material calculated in formulation as taken in ratio?

In liquisolid technology, carrier and coating material in ratio taken the add non volatile solvent then check angle of slide then how weight of carrier and coating material calculated to add in...

09 January 2021 9,589 5 View

Metabolomics Data Normalisation?

Hello, Setting: We have a rather large dataset consisting of somewhat 500 samples containing metabolomics data. The data was obtained by analysing dry capillary blood using HPLC methods, yielding...

04 January 2021 8,773 5 View

What is the best way to maintain constant concentration of a volatile organic liquid in an open experimental set-up?

The purpose is to keep a NAPL (eg Toluene) concentration constant in an open tank set-up.

27 December 2020 7,325 2 View