I am testing four different enzymes for activity on the same substrate. One of my lanes, on the far right, always runs lower, despite being the same material as the other three lanes. This has happened in multiple gels. What is happening and how can I fix it?
Michael Lusk
In case you are using gel electrophoresis, the apparatus may be generating a relatively low-potential electric field in the region of the right lane. Subject to the terms of any warranty on the apparatus, use a voltmeter to test the potential difference across each of the four lanes. In case you find significant differences in the respective potentials, ask the manufacturer to replace the defective component(s).
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