Hello everybody,

I have troubles with RNA isolation from Arabidopsis roots. When the amount of root sample is quite high, the simple Trizol method works quite good. However, I often need to isolate RNA from very low root amounts (something about 10 mg), and in such cases the isolated RNA is not just low in amount but it also have very poor quality, namely low 260/280 and 260/230 ratios and distorted shape of the absorption curve. I would be very grateful for the method of microscale RNA isolation from low amounts of Arabidopsis thaliana roots.

Thank you!