Anyone knows about using the microbial enzimatic activity for characterizing the trophic conditions of salted lake?
There is a significant relationship between ectoenzymatic activities (Esterase activity, Aminopeptidase activity & Alkaline Phosphatase activity) of heterotrophic bacteria and the trophic state index of the lakes. Esterase activity (EsA) can be assayed fluorometrically as an increase of fluorescein concentration in water
samples incubated with fluorescein-diacetate (FDA).Aminopeptidase activity (AMP) is measured fluorometrically as rates of 7-amino-4-methylcoumarin (AMC) released in the course of L-leucine--4-methyl-cumarinylamid hydrochloride (Leu-MCA) hydrolysis by the aminopeptidase present in water samples. Alkaline phosphatase (APA) activity can be determined as the rates of increase in fluorescence of methylumbelliferone (MUF) (365 nm excitation and 460 nm emission) resulting from enzymatic hydrolysis of non-fluorescent substrate methylumbelliferyl-phosphate (MUFP). The following references may be consulted for the purpose.
Hoppe (1983). Significance of exoenzymatic activities in the ecology of brackish water: measurements by means of methylumbelliferyl- substrates. Mar. Ecol. Prog. Ser. 11, 299.
Chrost et al. (1999). Fluorescein-diacetate (FDA) assay for determining microbial esterase activity in lake water. Arch. Hydrobio. Spec. Issues Adwanc. Limnol. 54, 167.
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