The nutrient analysis in fertilizer sample can be done by the following link

agra.ciat.cgiar.org/.../FERTILIZER%20ANALYSIS%20PROTOCOL.doc

Dear Tanuja,

The most popular method for determining Total nitrogen in Urea is The Kjeldahl method or Kjeldahl digestion (Danish pronunciation:  in analytical chemistry is a method for the quantitative determination of organic nitrogen in chemical substances like ammonia developed by Johan Kjeldahl in 1883.The Kjeldahl method was developed over 100 years ago for determining the nitrogen contents in organic and inorganic substances. Although the technique and apparatus have been modified over the years, the basic principles introduced by Johan Kjeldahl still endure today.Kjeldahl nitrogen determinations are performed on a variety of substances such as meat, feed, grain, waste water, soil, and many other samples. Various scientific associations approve and have refined the Kjeldahl method, including the AOAC International (formerly the Association of Official Analytical Chemists), Association of American Cereal Chemists, American Oil Chemists Society, Environmental Protection Agency, International Standards Organization, and United States Department of Agriculture.

Titration quantifies the amount of ammonia in the receiving solution. The amount of nitrogen in a sample can be calculated from the quantified amount of ammonia ion in the receiving solution.

There are two types of titration—back titration and direct titration. Both methods indicate the ammonia present in the distillate with a color change.In back titration (commonly used in macro Kjeldahl), the ammonia is captured by a carefully measured excess of a standardized acid solution in the receiving flask. The excess of acid in the receiving solution keeps the pH low, and the indicator does not change until the solution is "back titrated" with base.

Please find attached detail kjedahl method,

regards,

Prem Baboo

http://www.coleparmer.in/TechLibraryArticle/384

https://www.google.co.in/url?sa=t&rct=j&q=&esrc=s&source=web&cd=17&cad=rja&uact=8&ved=0ahUKEwiGq9n4_YHOAhVLvo8KHY44A1kQFghsMBA&url=http%3A%2F%2Fwww.sigmaaldrich.com%2Fanalytical-chromatography%2Fanalytical-products.html%3FTablePage%3D103218423&usg=AFQjCNFvz07DspbdA-_t0rwxhEyaJjz27w&sig2=_HMfb8zGaRHyuGIBPbrYDw

Take a known weight of urea and digest it in boiling H2SO4 in presence of CuSO4. Cool and make volume to 100 ml. Take 20 ml aliquot and distill using Kjeldahl apparatus (absorb in boric acid) and titrate against a standard acid.

Sushanth ji, Prem ji and Manoj Kumar ji, thank you all for sharing valuable information.

Thank You once again for your time. The info helped me to start the analysis in a right direction.

i want to know protocol the qualitative analysis of nitrogen

Is there any protocol for chemical analysis of leachates.

Thank you in advance

To analyze the total nitrogen content in a urea sample, I would typically employ the Kjeldahl method, which is a widely recognized technique for such analyses. Here's how I approach the process:

1. Digestion: First, I digest the urea sample with concentrated sulfuric acid, which converts nitrogen into ammonium sulfate. During this step, I carefully control the heating to ensure complete digestion.

2. Neutralization: After digestion, I neutralize the acid with a strong base, usually sodium hydroxide (NaOH). This step releases the nitrogen in the form of ammonia gas (NH3).

3. Distillation: I then distill the ammonia into a receiving solution, often a known volume of standard acid, which traps the ammonia.

4. Titration: The amount of ammonia is determined by back-titration. I use a known concentration of standard acid for absorption and then titrate the excess acid with a standard base.

5. Calculation: Finally, I calculate the total nitrogen content based on the volume of standard base used in the titration. The stoichiometry of the reaction is used to relate the amount of base to the nitrogen content.

By following this methodology, I can accurately quantify the total nitrogen present in a urea sample. This method is robust and reliable, provided all steps are performed with precision.

l With this protocol list, we might find more ways to solve this problem.