Hi,
Can someone suggest me a method of fixing cultured neurons for fluorescent staining and long term storage?
Would I not need to go for paraffin fixing since they are cultured cells?
Thank you Frida Gorreja . That is very helpful.
So if I go for formaldehyde fixation, would I be able to store the cells for long term?
Thank you very much.
Use either a solution of acetic acid (5%) and absolute ethanol or( 95%) paraformaldehyde. We currently use this method with success.
Thank you very much Philippe Poindron
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