How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How to extract NLR genes from a database?

Greetings to all, I am not a bioinformatics person, but looking for a method that help me extract all NLR genes from benthamiana, tomato and soybean databases.. any suggestions so I can use my...

08 February 2021 4,557 2 View

Why do high doses of humic substances inhibit the growth of corn roots? Is there a toxicity effect?

Length, volume, surface area and dry mass of corn roots treated with humic substances increased up to 13 liters per hectare and decreased with higher doses.

02 February 2021 2,190 4 View

Is corn fiber hydrolysis possible without the use of enzymes?

I dont have access to the needed enzymes for CF hydrolysis, so I'm wondering whether I can do the hydrolysis solely with the use of acids? Has it ever been done before?

08 January 2021 1,243 3 View

How can I reduce the viscosity of corn oil used as vehicle for administration in animal experiments?

Dear all, I am using corn oil as vehicle for subcutaneous injection and I want to reduce the viscosity of the vehicle (corn oil) to enhance the delivery of the main compound of interest....

18 December 2020 1,773 6 View

Are there any alternatives to Corning's Transwell inserts for Air Liquid Interface culture?

There is apparently a worldwide shortage (due to COVID) of these 0.4micron pore, polystyrene membrane transwells...Are there any companies that make suitable, good quality alternates as we are...

09 December 2020 6,771 2 View

What density do we best consider an experimental unit as a missing plot in Soybean and groundnut. Can someone help share related journals?

Many field experiments particularly for crop plants faces challenges from sowing to germination to phenology up until yield. Too much a few of these challenges includes birds infestation,...

18 October 2020 7,436 4 View

Effective method for removing precipitation of CSL for biomass production? ( I centrifuged CSL but after autoclave precipitation was formed)?

We are going to use CSL as a cost-effective nitrogen source for Lactobacillus fermentation in pH 6.3. We have examined the acid treatment method to lower the pH of CSL to 3.5; after that, we...

12 September 2020 6,784 2 View

How to sample maize leaves and transporting them for specific leaf area measurements?

Dear RG community, I would like to sample (cut-off) maize leaves in the field and transport them to the lab for SLA (specific leaf area) measurements. After a brief literature review, I could not...

18 August 2020 6,079 4 View

What systematic experiment will help determine whether or not a range of mutants with similar phenotype are derived from a common ancestor-cultivar?

Several soybean cultivars were mutagenized to produce many lines of M0, M1, M2 etc. In order to determine the genetic basis of an observed phenotype, an M0 level mutant was hybridized with its...

09 August 2020 9,224 3 View

How do I take destructive samples from a randomised complete block design without destroying the blocks or affecting statistical analysis negativly?

I am working on an experiment where I will be growing soybean in pots through all its stages in a greenhouse, I have a total of 65 plants in the beginning with 4 treatments and 1 control group...

06 August 2020 9,880 3 View