Hey there,

I am trying to set up experimental equipment to measure the diffusion coefficient of ATP and ADP in Cellculture Medium. I did some experiments before to measure some other metabolics of cells, but it was done with a self constructed two chamber system. Now i want to go "more professional" as it's for my phd thesis. Does anyone have experience with two chamber diffusion measurements or can give me a hint what kind of system would be the best?

I can set up my self, but right now, the membrane between the chambers is the biggest problem because the diffusion length is not given accurately enough due to not exactly vertical membrane poreways.

Thanks in advance.

Alex