May I seek your expertise in identifying this snake species?

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Can I use General Health Questionnaire (GHQ-12) to study psychological distress among flood affected individuals in 2018?

I would like to asses the psychological distress among August 2018 flood-affected individuals. Can I use GHQ-12 in 2020?. Because there is a time gap of 1 and half years after the floods, does it...

23 February 2020 9,731 3 View

Merging data is SPSS by variable?

I have two data sets A and B. Set A has 6000 cases but has interested variable V1. Set B has a variable V2. The cases are generic in set B (so cannot merge with Set A by case). I want to add V2...

31 January 2020 8,408 3 View

How far (Kms) that the Salt water intrusion takes place from the sea (Underground) towards the main land?

Suppose If I want to dig a well say near to the Arabian Coast (India), How far from Sea that I have to dig a well in order to avoid the salt water intrusion through coastal aquifers. I would like...

23 May 2019 2,859 1 View

Endothelium Derived Hyperpolarization Factors and significance?

I am interested to know if EDHF a primordial factor for relaxation? What are the physiological relevance and sources?Does EDHF observed in Conduit artery provides any significant impact in...

20 April 2016 7,710 14 View

Want to know best algorithm for Approximation algorithm and Distributed algorithm in Dominating set and Wireless Sensor Networks?

I want to know which algorithm is regularly used for Approximation algorithm and Distributed algorithm in Dominating set and Wireless Sensor Networks?

28 March 2016 5,044 1 View

Are soluble proteins active or inactive?

I am curious to know whether soluble proteins are active in circulation? or they become active after entering the extracellular space? I know it varies from protein to protein but would like to...

21 January 2016 7,774 3 View

How to check the micro and macro analysis report for welded joints ?

I tested but whether it is right or wrong I got the test reports from a testing lab.

27 March 2014 7,227 2 View

Sometimes for my PCR reaction I am getting a perfect desired single band and another time I am getting an extra faint band with desired band.

I am using a drosophila genome extracting a particular gene. I tried using fresh DNA, sterilized water and my negative control is perfectly alright. Each time i repeat my pcr the results varies;...

17 April 2012 2,026 2 View

In FM based passive radar how to Compressive sensing(CS) technique to improve range resolution, side lobe mitigation, target detection

Compressive Sensing is very popular technique to process sparse signal. most of the researchers applying CS technique for DVB-T/DAB or OFDM, GSM based passive radars whose bandwidth is small,...

01 January 1970 7,259 1 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Please suggest the website or link for the collection of data for (Cancer Incidance Rates in India)?

We are doing research for the prediction of cancer incidance rates increases in India upto next five years. Please help us to get the exact data for Cancer Incidance Rates in India. This will...

19 February 2021 8,478 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View