Hello Clarisse Brunet Avalos

I have not used Geltrex. But you can go through the links below in case you haven't come across.

https://www.tempobioscience.com/blog/matrigel-vs-geltrex-matrices-whats-the-difference/

https://www.researchgate.net/post/Geltrex-vs-Matrigel

Hope it helps.

Best,

Thanks Malcolm Nobre! I'll take a look at them.

Hi Clarisse,

I noticed your comment about matrigel shortage, have you considered Type-0 marine collagen from Jellagen? A non-mammalian Matrigel alternative.

We have comparative data we would be happy to share. We have stock on the shelf and would love you to try it, please DM one of our product specialists, [email protected] if you want to know more.

Kind regards,

Andrew

Hi! Another good alternative is plant cellulose -based hydrogels, They have been shown to be biocompatible 3D culture matrices in various applications, also for culture of e.g. midbrain organoids.

Here is a nice study about transplantation of stem cell based neural progenitor cells in vivo in nanofibrillar cellulose, GrowDex-T

Article An Engineered Three-Dimensional Stem Cell Niche in the Inner...

Some large well established groups ( Hubrecht Institute) have used Cultrex with very similar results. Whilst this was not specifically for cerebral organoids, it could be considered.Article Establishment and Culture of Human Intestinal Organoids Deri...

Hi! A Matrigel alternative:

10 branched poly(Arg, epsilon-Lys)-hyaluronic acid hydrogel (for 3D cell culture)

Xenogenic-free, chemically defined, highly reproducible and excellent biocompatibility Without crosslinker and organic solution Mimic the natural extracellular matrix(ECM). Proper mechanical strength Storage at room temperature No need to pre-treat prior to using with cell suspension To perform assays on isolated spheroids, dissolve PHA with iso-osmolar trisodium citrate. Mesh size 50, 100, 150μm

Break a leg!

denovoMATRIX has coated cultureware specifically for iPSC or MSC. The coating is xeno-free chemically defined matrix. You can find more on these on the U.S. distributor's website:

https://ilexlife.com/search?q=mymatrix&options%5Bprefix%5D=last