Troubleshooting pcr amplification with touchdown program: can anyone help please ?

10 July 2020 1,234 11 View

Is there a need of SeqScape soft ware and Variant reporter soft ware in MLPA data analysis process ?? Or it is only the coffalyser which is needed ?

Is there a need of SeqScape soft ware and Variant reporter soft ware in MLPA data analysis process ?? Or it is only the coffalyser which is needed ?

16 March 2020 9,640 1 View

Can enzyme characterization be done using RSM?

I want to characterize an enzyme.. In stead of one factor at a time procedure i was wondring if it can be done using response surface methodology... Is there any publications for enzyme have been...

30 December 2019 2,807 2 View

No title

04 December 2019 3,507 2 View

Hi, What is the size of NADPH enzyme in Dalton? It's molar mass is 745.4 g/mol?

I am working on a project involved drug metabolism by NADP/NADPH enzyme. I want to remove the enzyme from my reaction mixture. I am looking for the amicon centrifugal concentrator to remove that...

18 November 2019 1,141 3 View

How could I include an interface in Sdevice simulation of traps??

I am trying to simulate traps in TFET interfaces between oxide and silicon using sentaurus Sdevice. I included the traps in command file as follow: Physics (MaterialInterface = "Silicon/HfO2")...

16 September 2019 9,039 3 View

How to prepare a suspention of bacterial concentrate equal to1 x 106 /ml?

this suspention is prepared for DNA extraction

15 August 2019 8,871 3 View

I have old blood samples stored at 4 degrees since 6 months . What method of DNA extraction can I use to obtain high yield and good quality ?

I have some old blood samples stored at 4 degrees since 6 months . What method of DNA extraction can I use to obtain the highest yield and best quality ? . And If I am using modified salting out...

19 February 2018 8,986 5 View

What is the difference between lysis of bacterial cells using sonication and lysis by boiling with SDS-PAGE sample buffer?

Bacterial cells lysis sonication SDS-PAGE

04 January 2018 3,938 9 View

What are the main challenges of direct 3D printing with precious metals?

I am doing my dissertation on mass customisation and engineering of jewellery products. One of my aims is to find out why direct 3d printing in particular in the jewellery industry has not been as...

06 August 2017 6,323 9 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

Anyone found any research papers on solar furnace design calculations (for solar concentration)?

I am thinking about working on solar furnace with heliostats that concentrate sunlight and heat molten salt, but currently no luck with research. Not a lot of papers based on the subject. (check...

01 March 2021 5,023 5 View

How to size battery banks?

I am designing a small grid connected solar PV power-plant. As the battery's capacity is specified in Ah and demand in kWh. What is the best way to size the battery bank? I have battery price in...

28 February 2021 1,638 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View