Masslynx (Waters): How can i calculate the response for unknown compounds if i am using a calibration curve with the following response formular:
Area * ( IS Conc. / IS Area )
We may need some more information Matome, including your objectives and which instrumentation you are using. If you are trying to measure a response factor for chromatography, then this website gives a step by step guide: https://www.chromatographytoday.com/news/gc-mdgc/32/breaking-news/what-is-a-response-factor/31169.
If the compounds are truly unknown, then which chemical standard is being used for your calibration curve? It is not normally recommended to use a calibration curve for one compound to estimate the concentration of another since, for mass spectrometry at least, the ionisation potential for each ion is chemistry dependent i.e. the gradient of the calibration curves can differ from compound to compound. It is possible to estimate the linear range of response for any individual unknown candidate by using a dilution curve of a representative sample to estimate limits of detection.
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