Hi all,
there are some months that I've this 'problem'. A protocol of bacterial pellet lysis, ask for the addition of a solution 2M of sodium L-tartrate (I've started from the dibasic monohydrate powder).
I tried to solve it in distilled water, tris buffers and phosphate buffers (that are compatible with my lysis protocol), but I've always not solved reagent.
a collegue (chemist) suggest to sonicate or wait for an over-night dissolution, but I didn't 'solve' my problem.
does someone know the procedure, or if there is a critical step during the preparation?
thanks in advance for your help!
Giuseppe
The protocol clearly indicates a 2M solution that should be added 1:2 to the lysate (so, 1M final).
2% is more or less 1M, how do you solve it?
do you obtain a clear solution, without any precipitate?
thanks for your help.
Http://shodhganga.inflibnet.ac.in/bitstream/10603/774/9/09_chapter%203.pdf
please refer this article
in the paper, they start from a 2% stock (0.9M, in water) then diluted till 9mM, but they use sodium potassium tartrate instead of sodium dibasic (I checked, and it seems to be more soluble).
if there are no additional suggestions, I'll order the new form, and I'll try it. Thanks Suresh.
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