Hi,
Can anyone suggest the optimum level of magnesium ion intracellular concentration in E.coli?
Thanks!
In designing a clinical trial, it is necessary to consider a false pressure point for sham groups. Given that the main pressure point is around the lumbocostal region, what specific point or...
26 May 2023 8,250 1 View
Hi, I am trying to express a gene which belong to classical TA (Toxin-Antitoxin) module. Specifically, I am trying to express the toxin gene using pBAD vector in BL21 cells. Despite trying out...
08 March 2022 8,311 2 View
Hi, I am planning to tag a gene endogenously (in vivo) in E. coli for immunoprecipitation. Can anyone suggest an elegant protocol for this? Thanks!
29 June 2021 7,884 1 View
Hi, I am traying to optimize the expression level of my protein cloned in pBAD vector. Currently, I am inducing it at 0.002 w/v but see a significant overexpression of my protein. The current...
26 May 2021 6,335 2 View
Hi, Does anyone know of any plasmid where I can clone two genes under two promoters? Basically, I want to coexpress these protein together but with Arabinose inducible system. pETDuet,...
07 May 2020 8,328 0 View
Hi, Is there any way to prove that a protein is membrane anchored/peripheral membrane protein? Any simple trick or any technique will be helpful. Thanks
24 November 2019 1,152 5 View
Hi, Can someone please suggest which detergents to use in regular lysis buffer/resuspension buffer for E. coli cells? Please also suggest the optimum concentration to be used. Thanks.
24 November 2019 1,485 5 View
Hi all, I have a protein with conserved Threonine and one Lysine in the middle of the protein sequence. Being a charged and conserved amino acid across family domain of this protein, I have strong...
21 March 2019 3,201 4 View
Hi, I have cloned some three different constructs which are putative membrane protein of Mycobacterium tuberculosis into pET28a with N-term His tag. I tried to express them in BL21, Rosetta, C41,...
17 December 2018 8,644 2 View
Hi, Can anyone please highlight the difference between normal agarose and agarose low EEO? Any specification as to which to use for agarose gel electrophoresis? Is it that extraction of pcr band...
30 November 2018 6,802 3 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I conducted an adsorption experiment of arsenic on soil in the presence of different doses of silicon as competing ions to see the effect of silicon on arsenic adsorption and desorption. I took 5...
03 August 2024 6,500 3 View
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,761 5 View
What is human-computer interaction (HCI)?
22 July 2024 10,056 2 View
I am performing fluorescence experiments using a ligand to detect metal ions. I want to determine the Lowest Detection Limit (LOD) using the formula LOD = 3σ / K. However, I'm uncertain about...
19 July 2024 1,086 1 View
My research work is related to the calcination of limestone. I have a complex question and would like to consult with experts in this field. Could you please advise which kiln to choose (a...
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Suppose a material "A" has both tetrahedral and octahedral sites and we are doping another material "B" - usually an ion into it. How can we detect if the dopant has occupied the octahedral site...
17 July 2024 4,299 4 View
I need to plot cycle number vs. capacity with multicurrent density. please let me know if my steps are right or not as the below picture
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What are the opportunities and limitations for using digestate ion different crops and soil typs and how can its application be optimized - I have few of them listed and can be updated in this...
13 July 2024 2,431 2 View
I am working on an in-vitro model of hypoxia-reoxygenation (H/R) in cardiac cells (H9c2). Due to the unavailability of a hypoxic incubator, I am exploring alternative methods to create hypoxic...
30 June 2024 1,234 2 View