1.
Razin S.
Mycoplasmas are spherical to filamentous cells with no cell walls. There is an attachment organelle at the tip of filamentous M pneumoniae, M genitalium, and several other pathogenic mycoplasmas. Fried-egg-shaped colonies are seen on agar.
2.
Parija · 2009
Mycoplasma species typically show pleomorphism and occur as granular and ... M. pneumoniae does not produce fried-egg appearance colonies.
Dr S Kumar Prof. MAMC
DR R CHOUDHARY Prof. AIIMS
The culture technique are usually regarded as the gold standard method for
detection of Mycoplasma as it uses a selective growth media and incubation conditions to
grow any Mycoplasma present in the sample. Most Mycoplasma contaminates can be
detected on standardized agar like SP4 media or PPLO broth with subculture on PPLO
agar with the exception of M.hyorhinis. Colonies of Mycoplasma exibit a FRIED EGG
morphology when viewed under the plane microscope. For a distinctive result at least
incubation on an average of 28days is required
Figure 13: Colony of Acholeplasma laidlawii showing typical "fried egg"
morphology.
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