I am going to start the Lysimeter Experiment in which I shall grow rice crop and measure the losses of Ammonia (Boric Acid Trapping) and Nitrous Oxide (taking N2O sample by Syringes) from close chamber.

Literature shows that there is no issue of NH3 and N2O measurement from same chamber as there is no reaction of Boric Acid (used for trapping Ammonia) with N2O. Need expert guidance??

Secondly while using chambers for measuring Ammonia Losses from rice crop, I shall place the chamber in the lysimeter in such a way that rice plant is enclosed in the chamber for 2 hrs as losses are also from roots. I am going to use transparent chamber as there is no issue of light during sampling period for the plants. Needs further experts opinions related to the chamber used in rice crops?

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