If I were you, my best bet would be:

https://www.sigmaaldrich.com/catalog/product/sigma/s3382?lang=it®ion=IT

Even the Japanese scientists use it - that should say something about quality and reliability:

https://pathologycenter.jp/method-e/lfb.html

thank you very much for the suggestion.

FG

If you have recieved Luxol Fast Blue from Sigma (25 g is enough) then you should prerpare the following solution:

1g Luxol Fast Blue solve in 1000 ml 96 % Ethanol (ETOH); ad 5 ml 10 % acetic acid; mix well and filter this solution. You can store for several weeks.

For staining: after dewaxing your paraffin sections or defetting your cryo sections put the slides in to the 40 °C hot LFB solution for 16 - 24 h. After short rinses first in 70% ETOH and then in destilled water put them in 0,1% Lithiumcarbonate for 10 sec, 70 ETOH for 20 sec or until you can claerly differentiate gray and white matter. Short rinse in destilled water and now you can continue with cresylviolett as a counterstain (so called Kluever-Barrera-Stain). Good luck!