Hi everyone, I tried propagating the lentivirus packaging vector PMDLg/pRRE two separate times, and I got very low yield both times (
Same problem... To me, the main concern is pRRE. Have you sorted it out?
I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...
03 March 2021 3,661 2 View
I am exploring a number of SNPs, unfortunately although the test are ok, I am perplexed because my preliminary results are nearly all against what is reported in the literature. To confirm my...
29 January 2021 3,905 2 View
I am interested in publishing a recent scientific study (brief report) that may have some political ramifications. I am searching for a non-peer reviewed journal or publication platform. This...
04 January 2021 245 8 View
In every research paper, first author contribution is comparatively high. To tribute their work is there any index number for an author acting as first author in different papers?
15 December 2020 8,231 14 View
The age old question: How do you fix missing citations. IEEE Xplore has one of my papers with over 500 patent citations not including journal/paper citations. Google Scholar has the citations as a...
14 December 2020 9,588 4 View
How do I prepare a uniform azulene in MCH thin-films? I tried various methods(drop-casting and spin-coating) to get the azulene sample to stick to the quartz glass but did not work including using...
02 December 2020 296 2 View
I am interested on studies that combines business concepts and models in Public Health. I am also looking forward to communicate with respective researchers
30 November 2020 5,177 3 View
I know about a non-contact technique that can generate 2D maps of tiny internal currents--such as might occur because of microshorts--inside a pouch or prismatic cell. Potentially, this could...
21 November 2020 6,392 2 View
There are two websites for few journal with same journal title and same ISSN number. For example, There are two websites for the journal titled "High technology letters (ISSN: 1006-6748...
18 November 2020 7,650 6 View
Consider this vignette: during the COVID-19 pandemic a family are living in a big city where access to stable digital streams and affordable data bundles is not a problem. Confined to long periods...
22 October 2020 6,220 3 View
Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...
03 March 2021 401 4 View
Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...
02 March 2021 3,584 1 View
Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...
28 February 2021 4,868 2 View
In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...
25 February 2021 5,229 3 View
I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...
25 February 2021 3,030 1 View
25 February 2021 5,713 1 View
23 February 2021 7,826 2 View
Is it possible to implement FBM in a commercial FEA software package, such as FEMLAB, COMSOL, or open source software like Moose?
22 February 2021 2,968 1 View
Hi everyone. I am researching droguht effects on soil water drought. I am looking for already implemented functions in R or Python to estimate soil water content from precipitation,...
21 February 2021 2,084 1 View
For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...
20 February 2021 4,332 4 View