Hi everyone, I tried propagating the lentivirus packaging vector PMDLg/pRRE two separate times, and I got very low yield both times (
Same problem... To me, the main concern is pRRE. Have you sorted it out?
need to open an account to upload my published papers
01 August 2024 9,255 1 View
There are three factors involved in the activity, the substrate acting as a substrate, the substrate acting as an inhibitor and another inhibitor for which you want to measure the Ki.
08 July 2024 4,988 1 View
Can anyone help with a sample of Kraft pulp process lime mud (CaCO3) I need a sample of 250g to run R&D lab testing. I am based in the UK and I am struggling to get hold of some lime mud...
01 July 2024 9,217 0 View
I'm writing a letter of recommendation for a colleague and I'd like to mention how often her publications are cited by other academics.
28 June 2024 4,995 0 View
Recent research papers and journals on indigenous knowledge system
21 June 2024 6,358 1 View
l need an assistance on how to carry out a systematic review of causes and preventive measures against maternal mortality in Ogun State, Nigeria, 2018-23?
19 May 2024 9,103 1 View
We are using gaussian G-16 to calculate molecular properties. When I specigy in the rout line def2qzvpp ( #P B3LYP/def2qzvpp opt output=pickett) with RU (Ruthenium atom in the atom list) --...
10 May 2024 4,810 5 View
Metabolic disorders occur due to our proper habits of eating process and remineralisation in the mouth. Like food, water, and liquids, etc. Due to mutations in all our food in saliva, the...
22 April 2024 1,959 3 View
We have searched literature in Norway and North America trying to find evidence that brown or black bears preyed on Atlantic salmon (Salmo salar), despite the fact that these fish spawn in...
17 April 2024 2,262 5 View
As best I have been able to determine from reading literature, chimpanzees, gorillas, bonobos, and orangutans have been largely confined to tropical and subtropical forests, at least during the...
15 April 2024 2,568 0 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View
Hello, I am a master's student currently conducting lentivirus transduction experiments in Jurkat cells. The virus is not effectively entering the Jurkat cells, so I am planning to use an...
21 July 2024 4,676 4 View
> library(meta) Error: package or namespace load failed for ‘meta’ in loadNamespace(i, c(lib.loc, .libPaths()), versionCheck = vI[[i]]): there is no package called ‘numDeriv’
18 July 2024 3,536 0 View
I've been trying to clone my N-terminal inserts in the comercial pEGFP-N1 vector. Initially, I cloned my N-terminal insert into a pGEMT-easy vector to ensure that the insert digestion was done...
09 July 2024 4,277 1 View
i need to ask that when I transduce my HEK293T cells with Lentivirus, how much time will they require for transduction because when I add lentivirus and then change the media after 24 hours, my...
07 July 2024 2,946 1 View
Hello, recently i am an issue swith cloning where I am using the subclone method to insert my gene length is 1.5kb and my vector(pMAL-c6T) is 5kb I got the colonies which i screened through the...
04 July 2024 7,977 2 View
What are the reasons why we need to practice proper post-harvest handling including packaging, after harvesting fruits and vegetables?
02 July 2024 9,999 2 View
I am trying to clone a gRNA (23 bases) into the Crispr cas9 pDirect23A vector by using restriction enzyme cloning. I have performed a complete experiment, starting with plasmid isolation,...
02 July 2024 5,448 2 View