Hi Nikhil,

depends, which effect you want to study. Do you want to find an insulin sensitizer, insulin secretion enhancer, fat cell growth inhibitor, energy turnover enhancer, glucose uptake increaser?

Do you want to study effects in cell culture or just binding to specific recombinant proteins? What do you want to (or are you able to) measure: changes in protein phosphorylation, gene transcription, glucose uptake, insulin secretion, changes in metabolites?

Do you just want to do a general screening or do you already suspect a certain effect? Why only diabetes and not "everything" - like cancer, heart, lipids, alzheimer etc? I.e. trying to find "all possible" (and maybe impossible) targets. Maybe it makes sense to offer you compound collection to a company or institute to include it in a library which may be screened for various purposes.

Careful planning will be necessary, if not, it's easy to waste lots of time, manpower and money and, even more serious, neglect possible effects of interesting compounds.

I am also interested in this topic: What else can you do with novel compounds / structures you have synthesized for a certain purpose? I am working for a small company that focuses on a narrow field and which does neither have manpower nor money to initiate screening programs for possible drug or alike effects. How to find possible partners who might be interested in these compounds? How to deal with possibly arising issues about intellectual property, as usually, one doesn't file patents on these substances / syntheses, as long as you do not see a practical application that might at least reimburse the costs?

Hi nikhi

I am interested to do study about new herb drug for treatment of diabetic women. If you have new idea , i am ready fto cooperate in common research with you.

@wolfgang: Hi, Thanks a lot for your valuable suggestions and interest.

I am working in Medicinal chemistry and have no background of evaluation of drug. I am PhD student and my thesis topic is "synthesis of new antidiabetic molecules". So thats the reason why I am interesting in invitro antidiabetic testing.

I have synthesized new molecules and I want to do very simple, easily available (in India) invitro antidiabetic testings. If it works, I can contact expert in pharmacology field to evaluate molecules in details. So please suggest me in vitro antidiabetic methods. I think, now special kits are available. If you know about it, please forward information.

You asked.. Why only diabetic n not "everything".. I have answered you "why antidiabetic". I want to do other studies also if possible. Again, conditions are same.. Tests should be simple (as I am not expert in evaluation) and easily available. Please suggest me the methods.

You have raised very important topic. I also thinking in same way. Why shouldn't we evaluate our drug for all / many diseases? On 3rd may NIH centre, US aimed to match scientists with discarded drugs. please see the link (http://blogs.nature.com/news/2012/05/nih-centre-aims-to-match-scientists-with-discarded-drugs.html?utm_source=twitterfeed&utm_medium=twitter&utm_campaign=Feed%3A+news%2Frss%2Fnewsblog+%28News+Blog+-+Blog+Posts%29). First HIV drug was actually anti-cancer moiety. We can have big debate on this topic at research-gate to find best solution.

Now, Please suggest me

1. very simple, easily available (in India) invitro antidiabetic testing methods

2. other studies also if possible. Again, conditions are same.. Tests should be simple

@Farideh:

Hi, Thanks a lot for your reply and interest.

I am PhD student at Institute of Chemical Technology, Mumbai, India. If you have funding or have any idea about international collaboration, I will forward your offer to my Research guide for sure. I will also search for such funding.

I don't have ready idea about treatment on diabetic women but will search for sure.

Dear Nikhil,

the real challenge is to define "antidiabetic". If you just look at the different drugs available to treat Type 2 diabetes, you will see there are mayn different modes of action.

Another approach is to address the topic by "what may be tested easily", means by western blotting, ELISA and uptake of isotope labeled substrates:

You may check the effect of your compounds on the response to insulin. I'll try to dig out something:

1) If you have access to cell culture (any cell with an insulin receptor will do, as eg fibroblast cell lines, however, (primary) adipocytes, myoblasts or even myotubes are 'better' (results have wider acceptance), all preferably of human origin. Also leukocytes isolated from blood are an option (you may ask people who can provide them and do a collaboration)). After having done cytotoxicity assays (eg growth, MTT/XTT) to determine reasonable concentration ranges, add your drug and insulin and check the response by measuring PKB/Akt phosphorylation (western or elisa), later then the uptake of 3H-deoxyglucose and glycogen synthesis from 3H-UDP-Glucose. You may check the papers by Haring-HU and Klein-HH for plenty of detailed examples.

2) Suitable arrays: e.g. Qiagen recently has acquired a company that has developed qPCR arrays for mRNAs believed to be important in metabolism. If you have the hardware (qPCR machine), an array of about 90 reactions is about 500 USD. There should be other methods around to do similar approaches like 2D-DIGE (see the most recent paper in my profile) or Luminex or lots of DNA arrays.

A third option is to screen your compounds first in silico: Get hold of the protein structures of as many proteins as possible involved in insulin signaling and have a computer running docking analyses for potentially binding drug/protein pairs. In this way (which may be done very quickly on a current computer) you may narrow down the number of experiments and generate a priority list. Having the compounds and such a list, this might be a good option for a research grant going abroad and run the tests in a good suitable institution. For docking software, you may check http://pyrx.sourceforge.net/

HTH & have fun!

Wo

Dear Wo,

Thanks a lot!. This more than what I wanted. I will search such people near by and will go for collaboration as per your suggestion. Elisa n western blotting will work at our institute. Though we dont have qPCR machine, If I can collaborate with people, It is easy to handle.

About "everything" evaluation, I would like to start new topic in medicinal chemistry section so many can give thier suggestions.

@nikhil jadhav check this paper - http://www.ijrpbsonline.com/files/44-3265.pdf

Thanks a lot akshada.. It is really great!!!

hello nikhil

iam also searching for such low cost invitro tests for testing antidiabetic activity. plz suggest me if u find some useful tests.

hope you found the answer to your question and also useful tests..

Hey Manoj,

Please refer Akshada's previous comment.  The paper gives enough information of our query.

i am also searching for the same. please let me know if you get any link

Hello Nikhil,

You can go for alpha glucosidase or alpha amylase activity. You can also go for non-enzymatic glycosylation of haemoglobin assay but for that you need to collect the blood. 

Hi!

Akshada Khadpekar's link is not working. Can someone help me look for this article? I'm also looking for in vitro antidiabetic tests

Suggest me kit methodology