Hi, it's ANITH KUMAR RAJENDRAN, I am doing Ph.D @ SRMIST, Chennai. I am looking for TRBO vector to start my project. If anyone is working this vector and is it possible to share sample with us please kindly inform.
This plasmid is available from addgene for $75 US Dollars. Here's the link: https://www.addgene.org/80082/
I usually don't see path loss models for IRS consider polarization. But specific to mmWave application, the polarization of the antenna has some effect. Is there any research that has explored this?
08 January 2021 3,054 1 View
We would like to implement SDN based IoT secure application. Please suggest best open source Emulator / Simulator.
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dear researchers at present we are working in fire detection projcts ,i have a plan to implement the CNN with SVM for classification method. if you hav any best or recent techniques please let me...
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In our lab, we are working on a herbal extraction optimation so that we need to understand the total profile difference between optimized and unoptimized extraction. Which that we have multiple...
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I have been trying to check the supplementary file for this paper entitled "Transcriptomic and proteomic analyses of the pMOL30-encoded copper resistance in Cupriavidus metallidurans strain CH34"...
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Hi, I have a problem with cryosections while staining. Positively charged slides are used for cryosections, in spite of that during the staining process, the sections are washed away during the...
10 September 2020 3,767 3 View
I am going to conduct experiment on sunlight collection. First the sunlight is collected using Fresnel lens. Then the concentrated light is transported via silica optical fiber. Since the...
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As a part of my research work. i have obtained some machined surface using vertical milling and turning process. I want to check whether the predicted roughness and obtained roughness(Ra) are...
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I am collecting sunlight via optical fiber bundle. In this regard i need to make fiber bundle in which the individual fibers are to be stick together. What kind of adhesive is helpful to do this?
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I have two products (A&B) made of polyethylene (PE) but product "A" is produced from recycled PE and product "B" is produced from virgin grade PE. What is the willingness of the people to pay...
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Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...
03 March 2021 401 4 View
Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...
28 February 2021 4,868 2 View
When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...
25 February 2021 1,011 3 View
Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...
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For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...
20 February 2021 4,332 4 View
1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...
20 February 2021 2,370 1 View
i have a vector 1*512 size, i want to take the first value every 8 elements and put these elements at the end of the vector,how to do this on matlab ?
19 February 2021 2,352 5 View
Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...
17 February 2021 4,844 4 View
Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...
15 February 2021 4,353 3 View
Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...
15 February 2021 9,563 6 View