Hi all, I am doing a couple of germ free mouse experiments and have some issues with contamination likely due to spores in the hood colonizing my germ free mice. My current sterilant has not been tested for spores. Anyone has any suggestions on potential sterilants that will not corrode the metal in the hood but can kill spores?
Hi Janice,
Have you tried PREempt? You can try it for sterilizing your hood.
Hi Manoj, thanks for your answer. I just checked the retalier website for PREempt. The product information mentions it can kill a broad range of microbes but does not mention if it can kill spores. Do you happen to know if it can kill spores? Also, is it corrosive to metal workspaces?
Usually space/ air is sterilized/ disinfected with vapors of formaldehyde! The formaldehyde is poured in a wide surfaced Petri dish or tray and allow to fumigate in space over night. The spores become inactivated with this treatment.