How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for Metabarcoding?

I'm working on pollen DNA metabarcoding to identify the floral composition of honey. How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for the...

28 December 2020 9,132 1 View

Can anyone help in doing SEM of pollen?

Is there any institute in Kerala were v can do SEM studies.

11 November 2020 5,001 3 View

I need info on particular Convolvulaceae pollen, can you help?

Dear colleagues in palynology, paleobotany, and botany I desperately need information/papers on the pollen morphology of the following Convolvulaceae genera: Astripomoea, Calycobolus,...

14 October 2020 8,216 10 View

What is the best method for collecting and preparing pollen from insects stored in 70% ethanol, for visual identification?

Hello, I would like to visually identify and quantify the pollen morphotypes carried by insects which have been stored in 70% ethanol. The insects are Diptera (mainly Syrphids), and Hymenoptera...

20 August 2020 8,859 3 View

Is it possible to remove pollen from museum bee specimens?

I have recently extracted genomic DNA from some museum bees for sequencing, and from first look there appears to be some pollen DNA in the extract. I've had a Google but can't find anything...

25 May 2020 8,423 2 View

Can adult butterfly or moth feed on solid food, such as pollen?

Dear All, Can adult butterfly or moth feed on solid food, such as pollen? It is known that nectar mainly contains sugar but very low level proteins, lipids. If a butterfly only feeds on nectar,...

29 March 2020 6,211 3 View

Species that contains the grass allergen Phl p5?

Does anyone know in which species the grass allergen Phl p5 occurs? It was originally isolated from the grass species Phleum pratense so it must at least occur in that species. It stand to reason...

15 March 2020 8,703 4 View

How different do fossilised and fresh pollen look under the microscope?

I was wondering whether fossilised pollen looks very different from normal pollen when you use bright field microscopy? In particular, do the following change significantly: Morphology...

29 November 2019 1,269 9 View

When doing Scanning Electron Microscopy of pollen samples from basal angiosperm group, is it necessary to do acetolysis?

1. Will there be significant differences in SEM images of acetolysed pollen and pollen fixed in 70% EtOH? 2. This is to study the micro-morphology of pollen structure and ornamentation for...

10 November 2019 5,022 2 View

Is there a protocol to isolate germinated pollen from solid media for RNA extraction?

We are trying to isolate RNA from pollen of Phlox drummondii plant. We have cultured pollen on solid media where it germinates. We now want to isolate this germinated pollen to extract RNA. Does...

10 November 2019 500 1 View