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More Chiriac Matei Alina's questions See All

How is it possible to model a protein that consists of several subunits using a Modeller?

Good day, I am trying to model the GABA A receptor, with different subunits. I selected the templates with the highest percent of identity, but as a result of the modeling I got a protein with...

02 June 2024 5,489 2 View

How to make a proper topology file for CNT-aminoacids water solution?

My aim is to simulate cnt with aminoacids(histidine) in water solution. I`ve made two separate topology files(15 molecules of histidine with pdb2gmx command and cnt topology with x2top) then merge...

20 April 2024 1,760 0 View

Why is my Synechococcus sp. cyanobacteria losing its color and producing mats of biofilm, I suspect it might be either biofilm or some form of EPS ?

I use BG 11+ Media but with an alternative trace metal mix.

17 March 2024 9,253 3 View

My Strep-tagged protein doesn`t bind IBA Tactin XT 4Flow spin columns. Could you please advice how to solve the problem with bindning to resin?

My protein is UFM1 tagged with Twin-Strep tag. I double-checked and my tag is not cleaved off or hidden. I made purification with IBA kit (Tactin XT 4Flow high capacity spin kit) with subsequent...

26 November 2023 4,087 3 View

With Vina, why did I get an output file with a different ligand location than the coordinate I specified in the configuration file?

Good day, I created a grid using the Autodock tools and then using those coordinates and size I created a configuration file for Vina. But in the results, the location of the ligand do not match...

12 November 2023 2,831 2 View

Dear colleagues, Do we have research/researches demonstrating exercising related changes in children?

I am working in Paediatric Outpatient Physiotherapy department with children with Lower Limb problems. As a part of our help to children we are running 6 weeks strengthening groups. The question...

19 September 2023 2,570 0 View

Should I order necessarily azide free primary antibodies for IHC, if I have HRP conjugated secondary antibodies?

I was wondering if the amount of sodium azide in primary antibodies for IHC can affect HRP activity in secondary antibodies, as I wash slides several times between the primary and the secondary...

12 September 2023 9,464 1 View

Does anybody have a good working protocol for IHC (tryptase) in mast cells?

I am a medical doctor and I do not have any experience in IHC, only toluidine blue and HE staining. Can you please share a protocol with me? Thank you in advance

12 September 2023 7,596 2 View

How to interpret the following RMSDS?

Hi everyone. So I have performed MD simulations on desmond of my protein bound to a mutant peptide? My objective is to elaborate how the mutant peptide is inducing conformational changes in the...

21 August 2023 7,117 1 View

What to do when the protein structure has high RMSD due to loops?

Hi! I ran MD Simulations of protein structure through SCHRODINGER software. The protein of interest has a lot of loops. I am getting very high RMSD of protein after simulations. How to solve this...

06 August 2023 7,344 3 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...

02 August 2024 7,843 0 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View

Have you heard about Technological Surveillance on Oceanographic Research Vessels?

It is important to highlight that technological surveillance on oceanographic vessels is a collaborative effort in which different specialists work together to effectively leverage technology in...

31 July 2024 1,633 0 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

How can I find writers for drafting, submitting, and publishing papers?

Looking for paper collaboration writers： Collaboration Model 1: 1.1 Based on the chosen topic, complete the paper writing, select an SCI or SSCI Q1 journal, submit using my QRCID, and complete...

27 July 2024 8,965 1 View

How to determine method detection limit in an analytical method?

I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...

24 July 2024 6,592 5 View

How to determine LOD values?

I am performing fluorescence experiments using a ligand to detect metal ions. I want to determine the Lowest Detection Limit (LOD) using the formula LOD = 3σ / K. However, I'm uncertain about...

19 July 2024 1,086 1 View

Can any of you suggest how to find the detection limit.?

I am working on flourescence sensing of heavy metals using quantum dots.Can any of you suggest how to find the detection limit. Whether it is calculated from formula or we can get from instrument?

19 July 2024 7,411 3 View

Who wants opportunities for scientific cooperation?

Dear Colleagues, I hope this message finds you well. My name is Noor Al-Huda K. Hussein,and I am a researcher specializing in deep learning applications in genetic data analysis. I am currently...

18 July 2024 5,562 0 View