I have interfering values of microplate at 234 nm recommended for assay. Any suggestions or detailed article would help. The attached protocol is being used.
I did sample blanks, and the strange thing is that the results are indicating pro enzyme activity for a std Quercetin which per literature ought to have inhibited the activity? I was wondering if all microplates absord at that wavelength or it is maybe something specific to the plates that I am using. Thanks for your feedbacks.