Currently I am conducting a study on the process of producing animal feed pellets made from Maggot BSF using the LCA method. Are there previous studies that can be used as a reference?
I am quite confused about what formula to use to compute my sample size. I will be conducting a Sequential Explanatory design wherein my QUANT phase will make use of mediation analysis and my qual...
22 April 2024 5,588 6 View
If I understood correctly, there are some ways to test if a scale is an "interval type," such as Rasch analysis. However, it is not clear to me on what basis this distinction is made. (e.g. Is the...
29 January 2020 5,230 6 View
I am looking for an assay that does not need added tissue plasminogen activator (tPA) for its function. Thank you in advance!
15 October 2019 3,575 0 View
Hi, I’ve recently been looking to analyse phospho CREB expression in peripheral blood mononuclear cells (PBMCs). I’ve been having a lot of issues with pCREB levels after stimulation and I was...
10 May 2018 9,487 4 View
Currently, I will conduct a research on Polychlorinated Biphenyls (PCBs) in e-waste in DKI Jakarta-Indonesia. This research will be able to provide important information about the existence of...
12 December 2017 6,816 3 View
The R function centralityPlot plots by default variables in alphabetical order, but I am considering to sort them according to their domain. I already used the label option assigning...
07 September 2017 8,410 2 View
MFA is a structured material flow analysis and systematic waste management as a model elemental composition of waste and material management performance evaluation of recycling facilities and...
27 July 2015 6,210 7 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...
07 August 2024 5,177 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
How do living organisms play a role in the water cycle and why is nonpoint source pollution potentially more harmful than point source pollution?
01 August 2024 7,061 2 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...
01 August 2024 7,199 1 View
I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...
31 July 2024 8,309 0 View