I want to detect and isolate Listeria monocytogenes from blood samples of animals, for that I have been looking for selective media and I came across various sets of selective media used with pre-enrichment and enrichment steps. These media include, Buffered listeria enrichment broth (BLEB), university of Vermont medium 1 and 2 (UVM), PALCAM agar, Dominguez-Rodriguez isolation agar (DRIA), followed by sub culturing by using tryptone soy agar with yeast extract or in BHI.
Kindly suggest which media either out of those mentioned above or any other would be suitable for effective isolation and further sub culturing of the Listeria monocytogenes
your suggestions will be greatly appreciated.
Thank you
ALOA (agar listeria Ottaviani Agosti) is used in food samples (ISO 11290). L. monocytogenes colonies are differenciated from other listeria species since there is an halo surronding L. monocytogenes colonies
Listeria enrichment broth medium (Lovett et al., 1987)
Listeria aesculin selective base agar medium (Curtis et al., 1989)
Agar listeria ottavian & agosti (ALOA) medium (Ottavian et al., 1997)
Kindly, find details in attached file.
You could use ALOA (Agar Listeria Ottavian and Agosti) medium for presumptive identification of Listeria monocytogenes in a clinical specimen such as blood. In the medium, observe for an opaque white halo: it is a specific L. monocytogenes colony characteristic.
I suggest this attachment
http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=CM0856&cat=&c=UK&lang=EN
I suggest PALCAM agar with its selective Palcam Selective Supplement for the isolation of Listeria