the paper say that:
The CH4 gas (99.9%, v/v) from a compressed gas cylinder
(Nanjing Special Gas Co., China) was bubbled into
500 ml distilled water with a rate of 160 ml min-1 for
least 30 min at 25°C, thus reaching a saturated level.
The corresponding methane-rich water was then immediately
diluted with distilled water to different saturation
required. The contents of CH4 in fresh methane-rich
water (10, 50 and 100% saturation) were 0.13, 0.65, and
1.30 mM, respectively, and maintained at original concentration
for at least 12 h.
Kindly if i want to transfer the gas in an air bag and not bubble it into water and inoculate gas to my broth culture by a syringe, then how much volume of gas should i inoculate into 40 ml broth culture to get the concentration of 1.3 mM methane in the broth culture?
If you want to use 1.3 mM of GAS Methane you should talk about the volume of your culture bottle - volume of liquid and not the volume of liquid i.e. 40 ml.
And the concentration will change during the process so you will not have constant concentration throughout experiment.
The concentration mentioned in your reference is not if methane but of methane saturated water, so its not the same thing.
Thanks a lot for your reply.you are completely right. I know that this is the concentration of methane rich water measure by gas chromatography. I want to start with an approximate amount of amount gas that get closer to that concentration when bubbled by syringe into my culture because this direct flow from cylinder is difficult to be done under the required aseptic conditions. So I need just physical calculation of the amount of gas that came from the cylinder under this mentioned flow . I multiplied 160 by 30 =4800ml which sounds very large number and this drove me to think that about less than 4
500 ml methane gas partially be soluble in water and the remaining release during bubbling. So according to these ratios , I may bubble 380 ml methane gas from the air bag into into my culture to assure that the same ratio of methane will become partially soluble in my 40 ml culture. Is that right?
First I want to ask you what you mean with culture? Do you mean liquid media or liquid media + cells.
In case if it mean liquid media + cell, you should not try that, because cells will respond to the methane inflow which will be more than your desired concentration.
But if you mean liquid media, than also I can't say how much methane will be absorbed in the medium, because there will be various salts, which will change the solubility of methane in solution.
What I can suggest is to inject methane gas in your bottle which has cells growing in liquid medium. Use the complete volume of bottle for the calculation and keep the bottle on shaker. Replace the gas phase as often as you can lets say may be every week. Again, not to use molarity in this case, use % which will make calculation easier and practical. If you will use percentage, than you can use C1V1=C2V2 for your calculation.
Asad Ali Zaidi Sorry I did not understand what you mean. Yes, his calculation is right but it cant be used, if you mean the answer 384.
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