Hello Everyone,
How do we obtain the values of kinetic parameters from KEGG Database,for glucose metabolism?
I am planning to do a westernblot for the microglia marker Iba-1, using mouse brain extract . Please recommend a good antibody for westernblotting.
17 October 2018 1,090 5 View
i already have output for TF*IDF output inpycharm...i required to plot a graph term and TF*IDF and document similarity and qurery comparision with documents. please suggest me using python code in...
29 May 2018 3,375 4 View
I am writing code for multi-carrier data transmission over time varying multi-path channel in MATLAB. Mathematically , received signal can be represented as, y=x(t)*h(t,delay)+n(t) Impulse...
20 May 2018 943 2 View
i have to measure protein concentration of bacteria on polyethylene surface. for this i used coomassie brilliant blue solution in perchloric acid as an indicator. but acetic anhydride is not...
18 February 2018 6,826 2 View
I have done in detail Raman studies for charge transfer between few layer MoS2 and donor and acceptor aromatic molcules (like TCNE, MV etc.).In general FWHM increase with increasing conc. of...
23 August 2017 9,529 2 View
I am trying to develop an ELISA to measure antibodies to Hepatits B surface antigen in human serum.My detection antibody is an anti-human IgG-HRP. I getting a proper sigmoidal standard...
27 July 2016 1,392 3 View
I used a protocol mentioned below and it did not work. dissolved single colony with 100ul of buffer(200mM LiAc,100mM SDS) incubated at 70C for 5min Added 300ul of icecold ethanol and vortexed...
14 March 2016 9,756 3 View
How can I differ optimal guaranteed Cost Control with optimal control? Yurong Liu, Department of Mathematics, China.
04 October 2015 1,904 5 View
I wanted to measure rat mast cell preotease-1 activity. Is there any ELISA kit available ? I couldnot find one. Also, if I have to make own plates and do it, what is the best antibody that have...
22 March 2015 3,086 2 View
I am trying to extract exosomes from mcf7 cell line. After the sequential centrifugation step I calculated the numberof exosomes per cell based on the nanosight data. It comes to be very low (of...
17 March 2015 1,932 9 View
Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?
01 March 2021 3,422 3 View
I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...
24 February 2021 974 1 View
I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...
21 February 2021 5,379 2 View
Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...
21 February 2021 7,447 2 View
I am looking for something similar than Ingenuity Pathway Analysis (IPA) in which I can enter the proteins differentially phosphorylated and I can visualise the pathways these proteins are...
21 February 2021 8,923 4 View
Dear all, I'm trying to understand if a molecule of my interest (capable of inducing glucose-stimulated insulin secretion) determines an increase in intracellular calcium levels in the presence of...
16 February 2021 453 2 View
Dear colleagues, I am writing an article that compares the measured and predicted resting metabolic rate in elite athletes. However, to define a cutoff point to determine which equation is more...
16 February 2021 4,515 3 View
Hi! I am looking for some specific information in regards to the minimum amount of UV intensity required to begin the photo degradation process in polymers. I understand each polymer is...
15 February 2021 7,703 4 View
Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...
12 February 2021 1,386 3 View
Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...
07 February 2021 9,989 7 View