How can I assess incorporation of C13 and N15 into proteins by MS?

06 July 2014 4,430 3 View

200 vs 300 nl/min for LC-MS/MS in proteomics workflows?

Hi RG community, I run a Proteomics Core Facility with a few LC-MS/MS systems (Dionex U3000 nano LC system) running at 300nl/min flow rate on 0.1mm ID C18 monolithic columns with NewOjective non...

03 April 2014 2,889 5 View

Does biotin suffer reductive methylation??

I'm running a quantitative proteomics experiment on biotynylated proteins (using BioID approach, followed by streptavidin pulldown). I'm planning to use reductive methylation (formaldehyde and...

01 January 1970 1,285 2 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

No title

I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

How Can I clain a removal of a co-author?

I would like to make a correction in a co-author in your paper which is wrong. This name is also in other papers of Kenton Ko. Zey Ko is not a Co-author . The correct name is Zdenka...

24 February 2021 3,476 4 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Difficulty in debugging code to correct error - TypeError: '<' not supported ?

I am trying with a Unet code ontained from net ...in tensorflow. When trying to predict the segmentation result with the trained and saved model with metric " iou" using "CustomObjectScope"...

22 February 2021 5,254 1 View

How to perform Haldane correction on SPSS?

I was wondering if anyone knew how to perform the Haldane correction for SPSS (this is the definition of the correction -...

21 February 2021 2,882 1 View

absorption= 1-mag(S(FP1:1,FP1:1)*S(FP1:1,FP1:1))-mag(S(FP2:1,FP1:1)*S(FP2:1,FP1:1)) is it correct for absorption ?

hi everyone I am simulating a unit cell structure in HFSS using Flouquet port having two modes for absorption I have used this formula:- absorption=...

17 February 2021 9,178 2 View

How can I calculate the magnetic force applied from a magnet (no electrism)?

I would like to calculate the magnetic force on a Fe304 particle which is into a liquid solution . The magnet is above the solution , and there is no electricity. I found this formula : F = (V...

13 February 2021 4,770 3 View

What is the best way to do symmetry in ANSYS Fluent for flow between two concentric cylinders ?

I am working on Taylor Couette flow between concentric cylinders and the results are correct for the full case. however, I wanted to do symmetry case to improve the mesh. I have tried periodic...

11 February 2021 2,193 6 View

What is the ideal value of UACI for image encryption scheme?

UACI - Unified Averaged Changed Intensity between cipher images. Why some papers refer ideal value to be 33.33 and some refer it to be 33.46? Which is the correct value? (In reference to image...

11 February 2021 9,562 2 View