Alejandro Martin Cohen

4 Questions 6 Answers 0 Followers

Questions related from Alejandro Martin Cohen

How can I assess incorporation of C13 and N15 into proteins by MS?

Hi there, I'm working on a purified protein sample which is was expressed in bacteria in C13 and N15 media. I need to assess the incorporation efficiency by MS as this protein is going to be used...

07 July 2014 4,477 3 View

200 vs 300 nl/min for LC-MS/MS in proteomics workflows?

Hi RG community, I run a Proteomics Core Facility with a few LC-MS/MS systems (Dionex U3000 nano LC system) running at 300nl/min flow rate on 0.1mm ID C18 monolithic columns with NewOjective non...

04 April 2014 2,939 5 View

Just installed LipidSearch 4.1, but it doesn't have many of the deuterated internal stds. Do you know when the 5.0 version will become available???

Just installed LipidSearch 4.1, but it doesn't have many of the deuterated internal stds. Do you know when the 5.0 version will become available??? Unfortunately we cannot build custom IS in 4.1,...

01 January 1970 747 1 View

Does biotin suffer reductive methylation??

I'm running a quantitative proteomics experiment on biotynylated proteins (using BioID approach, followed by streptavidin pulldown). I'm planning to use reductive methylation (formaldehyde and...

01 January 1970 1,326 2 View