I'm conducting an online mental health survey. I only want adults aged 18 years and over to complete it. Is it appropriate to block under 18s?

I'm conducting an online mental health survey. I only want adults aged 18 years and over to complete it. Is it appropriate to block under 18s by having a "forced response to age and a minimum of...

16 July 2020 3,416 1 View

How to improve transfection efficiency of DREADDs plasmid in iPSC-derived NSC?

Hi, I want to transfect hiPSC-derived neural stem cells (Axol Bioscience) with DREADDs plasmids (pAAV- CaMKIIa-hM3D Gq, Addgene) using the Lonza 4D Nucleofector system. The protocol I am using...

10 March 2020 6,335 3 View

Half of my PCR bands are incorrect size?

I ran 35 PCR cycles on six gDNA samples using 18.5ul of a master mix (made up of goTag, two forward primer, two reverse primers, nuclease free H20) and 1.5ul of DNA. The primers are specific for...

22 April 2019 6,640 7 View

Does anyone have experience of tetrachoric correlation with missing data?

I have binary data that I want to run a factor analysis on in Stata. I can run this fine using tetrachoric correlation, but I have nearly 50% missing data, so I really need to impute the data. I...

18 June 2018 6,052 2 View

Factors that modulate the expression of Iba-1 in microglia?

Several papers talk about states (stressors) and pathological damage altering microglial behaviour and morphology and, as such, Iba-1 expression. However I'm struggling to find in the literature...

05 June 2018 8,096 3 View

How to strip fluorescent antibody staining from tissue?

11 October 2016 7,279 4 View

How to choose the right wireless chronic electrode for in vivo recordings in freely moving mice?

I am looking for a set of wireless electrodes to chronically implant into the amygdala of my freely moving experimental mice. I have a few wireless electrodes from Neuronexus, however the company...

29 June 2016 2,821 1 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View

Are there any candidates for an Einstein's bare cosmological constant?

It is commonly accepted that General Relativity has its own 'bare' cosmological constant that contributes together with the cosmological constant resulting from vacuum energy density for an...

23 February 2021 5,448 8 View

How COVID-19 has affected mental health?

The pandemic has led to depression and anxiety and its negative effects shouldn't be ignored. However, it has also led some people to become more resilient! What do you think? Thank you.

21 February 2021 4,733 19 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View

Can medicinal plants really play an inherent rule in the COVID-19 tackle?

Numerous scientific reports cited plant extracts with potential antiviral activity and even anti-COVID-19? Why these findings are neglected? I remember very well the warning released by the WHO to...

17 February 2021 2,141 9 View

How can I interpret a ratio variable?

Dear colleagues, I am running a regression in which I have the following regression FoodExp = 67.04 DepRatio +.....e My interest is in the interpretation of the coefficient on the dependency...

16 February 2021 2,058 2 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View