Smart VacPrep, anyone had problems with degassing?

12 October 2020 1,907 2 View

Anyone know an effecient method for transfection / siRNA knockdown in organ culture (retina)?

Hello everyone, I have made a nice protocol for culturing whole adult rat retinas in vitro. This is also called organ cultures or whole mount cultures. The retinas are sitting on a filter in a...

15 July 2020 5,300 1 View

Factors affecting precipitation of serum proteins?

Hello, In most modern protocols for metabolomics/lipidomics proteins are precipitated using solvents such as methanol, acetonitrile or isopropanol. After vortexing/mixing the next step is often...

28 April 2020 3,383 2 View

Protocol for FTIR analysis of activated carbons?

Want to analyse functional groups on activated carbons (biomass based). Is there a good protocol including sample preparation and instrumental setup?

02 March 2020 6,972 2 View

Personality. Enherited or socially constructed?

What is your take?

28 July 2019 7,443 17 View

DNA barcoding: is there a COI alignment that spans the Eukaryota?

Regarding the DNA barcode COI (cytochrome oxidase subunit 1), I'm looking for a multiple sequence alignment that, ideally, spans the Eukaryota. Is there any such alignment available for...

30 September 2017 506 5 View

How can you get rid of air bubbles (or blisters) on butyl compound used for inner tubes?

Looking for a method to eliminate air bubbles on butyl inner tube? there are blisters on cured butyl inner tubes, is this a compounding problem or machinery process?

16 September 2016 5,717 2 View

Should I use "locus", "gene", or "marker"?

20 March 2014 8,234 4 View

International business, further globalisation or backlash?

In the field of international business the trend has been a move towards globalisation and integration. However research has shown that lately most government policies have been of a protectionist...

01 January 1970 8,882 59 View

Ambivalence: Where to draw the line between the pathological and the non pathological

When it comes to Ambivalence, it can be a symptom of mental illness but it is also something we experience in our everyday life ( I certainly do, and my wife hates it) . Where would you draw the...

01 January 1970 6,670 9 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View