Has anyone had issues staining AAV injected tissue with immunofluorescence?

I am injecting the hippocampus with an AAV8-GLT1 viral vector. I noticed when I am staining with immunofluorescence the secondary antibodies (Ms 598) is sticking to AAV virally injected tissue...

19 March 2020 5,961 5 View

Can anybody help me with the protocol on how to detect protease activity in a gel containing Casein substrate?

I have just cloned and got a soluble expression(50%) of a thermophilic serine protease and did the heat treatment for purification at 70 and 80 degree with over 90% purity, and also have a...

15 March 2019 9,330 3 View

What are best purification buffers and their conc. that I can use to purify a 6xHis- tagged recombinant serine protease using Ni-NTA resin ?

I have just cloned and expressed a thermophilic alkaline serine protease in E.coli BL21(D3). The protease was expressed as a soluble protein (about 60%) with about 30kDa Mol. wt. (confirmed by...

27 December 2018 1,777 7 View

Can i cytospin cells that are already fixed and stained for IF onto glass slides for confocal imaging?

I plan to isolate breast cancer cells from patient blood samples and would like to fix and stain them in-situ, within the Eppendorf, and then spin them onto a (poly-l-lysine-coated) glass slide...

14 August 2018 3,354 3 View

Can MOLT-4 cell be stimulated with anti-CD3/anti-CD28?

I am looking for a cell line other than Jurkat cells that can signal through the TCR. Would this be possible with MOLT-4 cells or do you know of other cells lines that I could use?

07 June 2018 651 3 View

What is best temperature for the incubation of thermophilic bacteria on liquid broth for the purpose of DNA extraction?

I incubated my organism (thermophillic bacteria) on LB broth at 37 and 40 degrees centigrade for the purpose of extracting DNA from the organism but did not show any growth after 22hrs of...

05 October 2017 601 11 View

Are there any publically available DNA database for recA gene sequences to use in metagenomic analysis?

14 August 2017 1,969 2 View

Collaborative research on investigating changes of cannabis flower throughout curing?

21 May 2017 4,620 2 View

Is anyone aware of smart phone apps or programs to measure the raw acceleration signal from the phone?

Looking for a way to measure, record, and export raw acceleration from the smart phone's internal accelerometer.

30 November 2016 7,438 6 View

No title

05 October 2016 8,471 1 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View