I'm getting a weird UV spectra for pure acetone. The analysis were performed on Shimadzu UV-vis spectrophotometer however the graphs weren't good with noise patterns as seen on the image. Any help would be appreciated.
The spectrophotometer is not working properly, or it was not used properly. This "spectrum" is just noise. It could be that the lamp is burnt out, or there could be an electronic failure, or your blank has a very high absorbance. Try to measure the absorbance of water, blanking with water. You should get a flat baseline. If the spectrum looks the same as the one you showed, replace the lamp or call a repairman.
The spectrophotometer is not working properly, or it was not used properly. This "spectrum" is just noise. It could be that the lamp is burnt out, or there could be an electronic failure, or your blank has a very high absorbance. Try to measure the absorbance of water, blanking with water. You should get a flat baseline. If the spectrum looks the same as the one you showed, replace the lamp or call a repairman.
It is due to the blank having high absorbance (as mentioned above). Acetone has a UV cut off of 330 nm. Try solvent like hexane, water it should be fine. If you try DMSO the "noise" should be from 280 nm. https://sites.chem.colostate.edu/diverdi/all_courses/CRC%20reference%20data/UV%20solvents.pdf
If it's glass or quartz cuvette then it's most probably due to the high absorbance of the solution.acetone absorbs maximum at 275nm and quite susceptible to photolysis resulting in the production of radials.
If using plastic cuvette with acetone, it damages the cuvette surface resulting in complete opaque surface.
You must use a quartz cuvette for UV measurements. Glass absorbs strongly in the UV.
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