E. coli colonies may have an inusual pigmentation pattern when cultured on EMB agar?

As can be seen in attached images, we have cultured a pure E. coli strain on EMB agar, and the strain have grown over all the inoculated area, but only in certain portions of the plate the...

09 October 2018 9,414 6 View

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08 September 2014 5,767 4 View

Can I obtain cold-set calcium-induced whey protein gels using WPC80 instead of WPI?

Has anyone worked with WPC 80 in order to obtain calcium-induced cold-set gels? Reference publications: Barbut, S. (1995). Effects of calcium level on the structure of pre-heated whey protein...

09 October 2013 2,031 9 View

How should injection of n-alkanes mixture be done in order to obtain Kovats indexes ?

Interested in the determination of the Kovats retention indices for each compound seen in chromatograms, using a commercial n-alkanes standard mixture, after SPME-GC-FID of food samples to study...

03 April 2012 1,376 8 View

Following of nitrogen compounds consumption by yeasts cultured with peptones as nitrogen sources?

We are studying different parameters associated with the growth of a yeast in a culture media containing yeast extract, lactose as the carbon source, and peptones (e.g. casein peptone) as the...

01 January 1970 2,291 5 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View