I have shotgun sequencing data of oral microbiome samples from two groups and I want to measure the alpha (Shannon Index) and beta (Bray-Curtis Dissimilarity) diversities.

I have already made this with raw and square-root transformed data, but I only observe differences between groups with square-root transformed data

For what I understand most of the people calculate these diversities with raw (or rarefied) data, thus, I would be grateful if anyone could elucidate-me if it is ok or not, in this case, to use square-root transformed data.

Thanks in advance

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