Hi Sandeep

I think you will find the guidance at this URL: https://www.microscopy-uk.org.uk/mag/artdec11/ac-mount.pdf

Best regards, Ian

Check A Manual of Acarology by Krantz & Walter 2009

Hii Sandeep

The best way to make permanent slides of plant mites is mounting plant mites in Hoyer's medium after proper dehydration in alcohol grades. for soil mites especially Oribatid mites permanent mounting is not advisable. You can choose temporary mounting in lactic acid on cavity slides after proper dehydration and clearing in 1:1 lactic acid and ethanol and you can preserve it after identification procedures in 70% or 90% ethanol with a drop of glycerin in an eppendorf tubes.

I also suggest the book A manual of acarology by Krantz and Walter

With regards

ARUN A

Lot of Thanks to Ronald Ochoa, Ian F Burgess Sir for the guidance & also thanks to Arun A. for the clarified explanation. I have gone through research papers and found Hoyer's Medium Method is mostly followed.

Dehydration and clearing in lactic acid 50% (15 min) and ethanol 96% (5 min max.), and then slides mites in Abies balsamea. Or you can go to the book A manual of acarology by Krantz and Walter.

Hi Sandeep

Like Dr. Ochoa said, A Manual of Acarology is the most important book you should read first.

In addition, I recommend you to read this article "Neuhaus et al. 2017. Collection management and study of microscope slides: Storage, profiling, deterioration, restoration procedures, and general recommendations"

With regards

Jhih-Rong

Thanks a lot Dear Jhih-Rong Liao . I will Surely go through this article you mentioned.

I am also in agreement with dear colleagues about using the manual of acarology by Krantz and Walter. This is a detailed book which we can refer in all aspects of the science of acarology.