Isotype control in IP, WB and Flowcytometry

01 January 1970 0 5K Report

Hi all,

isotype control seems usually omitted in WB, intentionally unused in Flowcyto, but necessary in IP...

I think paper concerning Flowcyto have clearly states the reason discarding isotype control--- impossible to make sure isotype-control-generated and antibody-generated FcR background staining match with each other, besides inability to account for spillover

But I believe expertise is still needed to illuminate the difference in the role of isotype control in IP, WB and Flowcytometry, and any comments are welcome.

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