QPCR considerations in analyzing samples from the field?

I am attempting to plan a qPCR experiment in which I evaluate the expression of multiple genes in various brain regions of fetuses, as a function of location in utero (intrauterine position,...

31 October 2020 5,788 1 View

What purity grade of PEG-300 and NMP should be used to dissolve drug for in vivo use?

Hi. I need to dissolve a drug to be given to mice by oral gavage. Based on a paper I found, the drug should be dissolved in 10% (vol/vol) NMP/40% (vol/vol) PEG-300/50% (vol/vol) water. Looking...

16 August 2020 8,685 2 View

Question for psychtoolbox users. Divided the screen?

*Question for psychtoolbox users* I using one screen. The function "flip" flips all screen. Can i divided the screen to two areas of "flip"? I have two stimuli with different time display flipping...

10 August 2020 4,828 3 View

Which mouse strain is best suited for HCT116 xenograft experiments?

I would like to perform a xenograft experiment with HCT116 cells on immune compromised mice. I was wondering which mouse strain would be best suited for such an experiment?

05 August 2020 9,437 3 View

What restrictions or guidelines do you know pertaining to Wind Turbine Noise in your country or others?

I'm looking for any kind of legislation, zoning, best practices, etc. either required or recommended by the state, the EU/other federation or local government.

17 January 2020 4,987 2 View

How to conduct moderation model in a mixed design?

I have a mixed model in which I have: - within independent variable with three levels (emotion regulation strategies) - between independent variable, continuous (fear of height questionnaire...

24 May 2018 4,107 2 View

Can any one give me a protocol for beta gal ONPG assay for AML12 transfected cells?

I have a protocol which we used a few years ago with 3T3 cells. With the AML12 cells I don't get any color. Any suggestions? Thank you.

30 October 2017 1,749 1 View

How to increase the yield of islets isolated from RAT?

01 February 2016 3,138 17 View

Advantages and inconveniences using adjusted area under the curve (aAUC) to study tumor growth ?

Hi ! I would like to know if there is inconvenients in using aAUC to stydy tumor growth in xenograft mice ? I also would like to know if bootstrap method is good to estimate a confident interval,...

05 January 2015 1,787 3 View

Protocol for fatty acid extraction from mammalian tissue - can anyone help?

I need to extract and identify the fatty acid composition (with GC-MSMS) of several tissues harvested from mice.

22 October 2013 1,414 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

How can Gamma Ray API units be converted to CPS (counts per second)?

Oil and gas companies run gamma ray tool and take readings in API units while Gamma ray is also noted in cps, so how these units can be converted into each other?

24 February 2021 7,545 2 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Onset of Photo Oxidation in Polymers?

Hi! I am looking for some specific information in regards to the minimum amount of UV intensity required to begin the photo degradation process in polymers. I understand each polymer is...

15 February 2021 7,703 4 View

How to determine the drug loading and the drug release capacity of a polymer micelle using UV spectroscopy?

Currently, I am working on PLA-PEG copolymer micelle for the drug delivery system. I have seen several papers where some researcher talks to centrifuge the drug-loaded micelle to separate the...

13 February 2021 5,822 7 View

How to calculate ultraviolet dose in Gy or J/kg?

Currently I'm doing a literature review regarding the comparisons of different types of radiation such as uv, electron beam and gamma radiation. But I'm having a difficult time converting uv unit...

11 February 2021 7,642 3 View

What is the UV-vis spectroscopy and HPLC UV detector?

I did spectrum scan to levothyroxine sodium which show highest absorption at wave length=230nm which the absorption =0.136 and it's absorption at 225nm =0.047 but when Injecting it on HPLC...

09 February 2021 1,631 3 View

What is the maximum wavelength and absorbance for dodecyl trimethylammonium bromide?

I am measuring the absorption of dodecyl trimethylammonium bromide surfactant using UV spectrometer. I am trying to set the wave length range for the experiment. Please does anyone have a idea of...

07 February 2021 7,720 1 View