Strategies for short hairpin RNA interference cassettes?

In your experience, how much does the distance between the U6 promoter and the annealed -short hairpin - oligonucleotides affect the RNAi cassette expression? How to clone a shRNA sequence...

16 January 2021 9,418 2 View

Why battery always retain the same value of power?

Dear all, I have been testing a model that is in the Matlab library (https://es.mathworks.com/help/physmod/sps/ug/simplified-model-of-a-small-scale-micro-grid.html), this is named "power...

10 November 2020 2,117 3 View

How does methylene blue fluoresce under dark UV?

Will it show as blue color under dark UV light (say 390 nm to 400 nm range) ? Does it degrade over time under long exposure of UV light (i.e. losing its fluorescein characters) ? What...

23 September 2020 9,752 2 View

How to avoid blockages of centrifuge filters when filtering polydisperse liposome samples for separation of free hydrophobic drug?

I'm using ultrafiltration for the separation of free curcumin from liposomes. The tubes are 1ml 0.45um cellulose acetate filter tubes (Corning Spin-X) and the centrifuge I'm using is the Thermo...

01 September 2020 8,875 2 View

How should private health-care systems be improved to guarantee everyone's right to health?

COVID-19 pandemic has shown big social inequalities in countries with private health-care systems. Governments must pay greater attention to the right to health, that every human being must have,...

31 May 2020 7,357 23 View

I'm trying to set up a TR-FRET experiment in my lab but I'm having some troubles. Could you give some tips?

I'm working with an his-protein and I'm trying to obtain an IC50 between the protein and the biotinylated substrate and a known competitor. But the experiment is not working. I will add the...

20 April 2020 4,485 3 View

What benchtop high pressure homogeniser for downsizing liposomes would you choose and why ?

Emphasis on: Minimising drug and lipid loss Minimising number of passes Achieving unimodal distribution Maintaining high encapsulation efficiencies Ease of use and maintenance

24 November 2019 1,946 2 View

How to quantify drug uptake of each cell line of co-cultured cells?

22 October 2019 3,153 3 View

Measuring presence and immersion: Within or between groups?

Hi, I am currently working on a project where I am looking to measure variables such as sense of presence, immersion, and realism using a self-report questionnaire. Participants are to be divided...

11 July 2019 943 1 View

Does anyone have a protocol of loading liposomes with doxorubicin?

I'm intending to load liposomes with doxorubicin but, i don't want to use the transmembrane pH gradient method, could there be other better method of doing the loading?

02 April 2019 4,967 2 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

What is the best and affordable method to make and distribute a survey?

What is the best and affordable method for higher education student to build and distribute a survey in the United States of America with consideration of the following points: There are no...

23 January 2021 9,342 2 View

In this winter, the trend of death and infection rate due to covid-19 in Bangladesh is much lower than USA or other 1st world countires, why?

According to the opinions of experts the casualties caused by covid-19 would suppose to be increased in winter. Similar trend is seen in the case of USA and other 1st world countries....

14 January 2021 2,146 2 View

Are there only winners in the world of exism?

BREXISM finally is over and they got a trade deal with europe now, but exism left the UK poorer according to the news Brexit is finally done. It will leave the UK...

23 December 2020 4,071 6 View

Follow up tests to explore interaction effects in a multiple logistic regression: can someone help explain my contradictory results?

I am conducting a logistic regression in order to examine whether there is an interaction between the number of stressors participants were currently experiencing (between 0-9) and their current...

20 December 2020 1,043 9 View

How is chemistry taught in schools in the United States?

How is chemistry taught in schools in the United States? A comparative study of high school chemistry education

10 December 2020 9,972 3 View

Can consensus metal price forecast be effectively used in the mining industry?

At the beginning of 2017, Donald Trump was about to be inaugurated as the next United States president. In anticipation of President Trump's policy changes in the United States, with possible...

28 October 2020 243 1 View

Cann you fix my institutional affiliation?

I am told my articles since May 2008 are listed by RG as "Salem State University." That is incorrect. All of the articles since May 2008 should be listed for "Salem University." I have never...

27 October 2020 811 6 View

The most of Physicists, who worked in the field of Statistical physics, committed suicide. Is statistical physics really a killer subject?

Tragically, in 1906, Boltzmann committed suicide and many believe that the statistical mechanics was the cause. He provided the current definition of entropy, interpreted as a measure of...

19 October 2020 9,482 18 View

Is Cucujus clavipes (Coleoptera: Cucujidae) legaly protected or included in the Red Data List/Book in any state of the USA or Canada?

Most (especially European and Asiatic) Cucujus species (Coleoptera: Cucujidae) are known as rare or threatened or even endangered. Is the same situation in case of the American Cucujus clavipes...

15 September 2020 9,190 4 View