i have developed a bioinformatic tool (Link: geltowgs.uofk.edu). that compares PFGE gel image analysis results to mathematical models of band sizes derived from WGS (FASTA files). I have suggested a new algorithm to count DNA fragments that co-migrates across each lane. We are about to publish ower work (Research gate DOI: 10.13140/RG.2.2.32752.76806) . The attached file illustrate our method.
thank you
@Moussaab Belarbi
thank you very much for your answer. that tool predicts a PFGE pattern from WGS but my tool compares a real PFGE image analysis results to the models derived from WGS sequences. We had to figure out a method to calculate how many different fragment accumulate in each band and we successfully found that method(described in the attached file).
sorry, the uploaded file was an old draft. The attched document is the complete one
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