To study the antifungal effect of plant extracts, you need:

1. Preparation of extracts.

2. Prepare the stock solutions of your fungi with convenient concentration (10p6 to 10p8 spores/ml).

The antifungal tests can be realized by radial growth or agar diffusion (wells, disks).

For radial growth, you can mix the extract with the medium at different concentrations. Then spread 100µl of stock solution of spores.

For agar diffusion method you can prepare disks with different quantities of extracts or put 20µl of extract dissolved in solvent (ex. DMSO) with different concentrations in wells. Then spread 100µl of stock solution of spores.

Don't forget to realize the control tests: effect of solvents, effect of known antifungal compound.

3. Select the bioactive extracts for further tests (bioautography), fractionation and isolation of bioactive compounds (chromatography, TLC, spectrophotometric and spectroscopic analyses).

Don't hesitate if you need more informations

Good luck!

Plant pathogenic fungi will behave any other fungi. So there are no special culture conditions for plant pathogenic fungi. You can follow the usual procedure followed for studying the antifungal activity of extracts. NCCLS broth dilution methods are considered to be standard for studying anti-bacterial or anti-fungal activity. If you want to publish in an international journal your work, you must follow NCCLS method.

Wish you all the best.

Dear amel

You can use the usuel procedure for antifungal and antibacterial activities. It's better to use broth dilution method for both screening tests and determination of minimal inhibition concentration.

Best regards

You can choose either Well diffusion method or Disc diffusion method.