From the references, I found that the NK percentage in the liver of B6.Rag1-/- mice was improved. How about the CD3+ cell percentage compared with normal B6?
Hi all, my experimental XPS results shown that my C3N5 sample consists of N-H bond, hence in this case I should incorporate the N-H bond into my DFT modelling. However, I do notice several papers...
07 August 2024 8,414 2 View
I want to draw ph diagram using Engineering equation solver EES for refrigerant R134a and R1234fy and some other. I don't know how to draw the multiple ph diagram on same dome or overlapping to...
30 July 2024 647 7 View
Hi there, I'm new to modelling with comsol. I would like to ask if it is possible for comsol to output linearly polarized (LP) modes? I tried modelling a simple single mode fiber with an enlarged...
23 July 2024 4,019 3 View
I am trying to drop cast 2D Materials on Glassy Carbon Electrode for Cyclic voltametry and EIS purpose. What would be best binders to use?
23 July 2024 5,776 2 View
I am now developing a python module for ms2 database searching, would like to realize a function that similar to what Xcalibur did, choose multiple mass spectra and get an averaged spectra. But...
22 July 2024 3,975 1 View
I use a DISCO DAD323 dicing machine to cut my sample,which has 1-2 micrometers SIO2 on its top.However,the SIO2 is broken and fall apart everytime.I tried different cutting speed but didn't work....
20 June 2024 1,131 0 View
I am doing DNA FISH on mice stem cells to detect an endogenous gene location. The expected result should have two signal spots, representing the two copies of the allele. However, what I see is...
06 June 2024 941 1 View
Recently I came across the Zaltman Metaphor Elicitation Technique(ZMET),a qualitative technique that uses metaphores. Definitely, it is new to me, howeve, it is interesting and relevant for my...
06 June 2024 8,336 0 View
I have modelled a refrigeration system using finned and tube heat exchanger and adopted the tube by tube method. I chose the R134a as a baseline refrigerant and developed the model according to...
30 May 2024 8,152 12 View
Hello everyone, I would like to culture muscle cells, specially Human Umbilical Aortic Smooth Muscle Cells (HUASMCs) in DMEM. There are various papers out there where this has been done, however,...
28 May 2024 1,398 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View