Does Promega's Passive Lysis 5X Buffer contains anti-protease and anti-phosphatase components?

Hi everyone, Do you know if the Passive Lysis 5X Buffer that Promega produce has these components? I always use it to lysate transfected cells and measure lucierase activity and now I'm wondering...

03 September 2018 1,322 1 View

QRTPCR amplification of target with low GC content?

Hey everyone. I have a question. I'm doing the in vitro silencing of a small RNA of 79 bp (a snoRNA specifically), and I need to design a protocol for the detection of the known-down of this RNA....

26 December 2016 6,061 2 View

How can I measure the total area of shadow protected by the vegetation and buildings in a city by using multi-spectral imagery?

18 October 2015 9,586 3 View

Where can I find (free download) a mass spectral database for fatty acid analysis?

NIST08 and NIST08s database installed is not enough for FAME analysis in GC/MS

10 November 2014 171 2 View

How I can determine Quantification and Detection limits in PAH analysis (GC/MS)?

Signal of analytes is zero in blank samples...(not detected).

31 December 2013 1,024 19 View

Which solvent has the best affinity with organics coming from biomass pyrolysis?

I tried bubbling it in acetone, but it does not retain organics. The solution is then analyzed by GC/MS but only acetone and its derivatives are detected.

05 June 2013 2,532 2 View

How to achieve good adhesion between metal or ceramic support and catalyst?

The idea is generate an high-temperature resistant catalyst.

03 April 2013 8,136 3 View

How to determinate LOD of CO2 by GC-TCD?

Several calibration levels (from 0.1% vol to 65% vol) of CO2 in the air were measured, but the response of the detector is non-lineal. Mixtures by mass flow controllers were prepared and the total...

02 March 2013 9,979 20 View

Does the geocentric theory turn out to be non-scientific versus heliocentric cosmological theory?

From the look of the demarcation criteria Does the geocentric theory turn out to be non-scientific versus the heliocentric cosmological theory? Is science no longer a vision surpassed by another?

01 January 1970 2,550 8 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

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01 March 2021 3,905 5 View

How to Balance Bench work with Reading and Writing?

I am a second-year grad student. I am finding it difficult finding time to read literature and write (things like qualifying exam, thesis, grants). I find myself so busy with bench work that I...

01 March 2021 3,325 4 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View