HPLC: how to calculate violaxanthin area if it overalps with neoxanthin?

Does anybody know of, or have a method suitable for HPLC analysis that allows to separate violaxanthin from neoxanthin? I want to quantify the xanthophyll cycle (violaxanthin, antheraxanthin and...

21 January 2021 5,775 5 View

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07 December 2020 6,382 2 View

How does the His-Link Protein Purification Resin (Promega) perform?

We have always used Probond (Invitrogen) but Promega resin is cheaper. I was wondering how the Promega resin performs, to see if it is worth to change brands. Thank you!!

18 October 2020 4,351 3 View

How should I approach a Multilevel (2-1-1) Mediation Model with Categorical IV in SPSS or R?

Hi, In my study I have to test a mediation hypothesis in a multilevel (2-1-1) non-longitudinal model, with a categorical IV. I have access to R and SPSS, eventually to MPlus as well. As I...

30 January 2020 2,111 2 View

How do Lexico-semantics features of Nigerian English affect secondary school students spoken English?

What are lexico semantics features of Nigerian English and how do they affect the spoken English of secondary school students?

28 January 2020 9,712 2 View

Can anyone help me with the detection of amyloid beta by western blot?

Can anyone help me with a working protocol for amyloid beta detection by western blot? I have extracted the whole proteins from the cells. For the Western blot I used a Precast gel 4-20% (in SDS...

25 November 2019 3,217 4 View

How to open pictures taken by using InCell2200 (file xdce) in ImageJ?

Hi everybody, I have problems in opening a .xdce file. The file is supposed to contain several series of images taken with the microscope InCell 2200. I tried to open the file by using either...

03 September 2019 4,096 2 View

Does the CellROX dye detect intracellular H2o2 or ROS in general?

Hi, I am looking for a dye to detect H2o2 in living cells that is not fluorecent in green because my cells are already GFP positives. Does anyone have any suggestion? I found the CellRox dye but I...

04 October 2018 4,770 3 View

How can i properly select the solvent to perform an extraction or to prepare a column?

I would like to attach a carbon triple bond to a benzaldehyde ring and to do that i am trying to perform a williamson reaction: https://en.wikipedia.org/wiki/Williamson_ether_synthesis. The two OH...

11 September 2018 4,206 3 View

Gene sequencing to check a SNP?

Hello, I have two cells lines: A375 and Mel-ST. This last one is an immortalized cell line from primary melanocytes. The BRAF gene should be mutated with V600E mutation in A375 cells but not in...

21 June 2018 8,561 6 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Could not amplify CD28 and CD3z ectodomain from cDNA of Jurkat Cells ?

For my experiments, I have to express ectodomains of human CD28 and CD3z. As a source, I extracted RNA from Jurkat cells and verified the quality of RNA on the gel. I, then, prepared cDNA using...

23 February 2021 7,051 4 View

Why does the sum of reaction forces not match the force component of a Free Body Cut in Abaqus?

I am using Abaqus to simulate a plate with in-plane loading. The boundary conditions allow the right edge to move up and down, the displacements on the left edge are fixed. There is no rotation...

23 February 2021 711 3 View

What effect does the large temperature difference between the two primers have on PCR, including real time PCR?

What effect does the large temperature difference between the two primers have on PCR, including real time PCR? If I did not get a result from real time PCR, is it possible that my problems are...

21 February 2021 4,094 7 View