Reusable CRISPR/Cas9 vectors
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Hi, I am confused with the calculation regarding the BOD5 for seeded dilutions using the below formula BOD mg/l = (D1-D2)-(S)Vs/P (As per standard methods). Could any one of you please explain...
03 June 2024 4,732 2 View
Hello I am trying to simulate 2dof cylinder for VIV analysis and I used an FDM method but the cd and cl values i got are so high and the damping is not coming into picture i tried and modified the...
17 April 2024 4,597 0 View
Please suggest how many rodents required for any study wild type rodents
04 April 2024 4,483 0 View
Can someone provide Arruda-Boyce(8-chain model) UMAT code for Abaqus?
12 March 2024 2,854 0 View
I can delete the elements, but can we also delete the part when the strain in an element of the part exceeds some value?
06 March 2024 1,245 3 View
I am looking for exact copy number of pET28a plasmid. A citation would be great. Literature search only shows that it is low copy vector, but I haven't found any papers that mention the exact copy...
26 February 2024 2,359 1 View
Hi everyone, I would like to seek some answers from you for my question above mentioned. If you know something let me know. Cheers
23 February 2024 2,397 2 View
In this research, I propose a visionary approach aimed at cultivating a united and forward-thinking human generation dedicated to ensuring the survival of our species. The strategy involves...
30 December 2023 2,085 1 View
Please see the attached pdf for the EDX scanning results. I would like to know whether the nanofiber in the SEM image contain silver. Thank you. BRs, David
17 December 2023 2,994 17 View
research papers for initial level gear train design is
13 December 2023 3,065 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,573 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
