Greetings!!!
In my work, i have to identify the Streptococcus agalactiae, S. dysagalactiae and S.uberis. Some of my colleagues suggested that it is easy to differentiate these three in blood agar but very difficult to prepare blood agar by yourself. So try to find out some other alternative to differentiate these three. Then i came to know about Edwards medium (also requires blood supplementation) for S. agalactiae. Is there any selective media available to differentiate these three organisms (>80-90% specificity) other than blood agar?
Thanking you
hi thulasiraman
S. agalactiae and S. dysgalactiae can be differentiate from S.uberis on the basis on biochemical test as later is mannitol positive test whereas S. agalactiae and S. dysgalactiae can be differentiated on the basis sodium hippurate test which is positive for S.agalactiae.
however if you are having pure culture colony of these bacteria you can also go for latex agglutination test based kit differentiation manufactured by Himedia. I used this along with PCR assay and found it highly specific.
Follow this article for even strain level identification of Streptococcus species.
http://link.springer.com/article/10.1007/s12088-015-0561-5
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