Hello, I wanted to do the quantification of protein by using Bradford reagent, however, since I don't have the Bradford reagent, I tried to make one. But I am short of phosphoric acid, is there any known substitution to replace phosphoric acid?
Hi,
Recommend acid is only phosphoric acid. But you try monobasic potassium phosphate or monobasic sodium phosphate and HCl solution pH 1 to 1.5, it just thinking.
Dear all, may be trying another method is your choice. Please have a look at the following links. My Regards
https://doi.org/10.1016/B978-0-12-802823-0.00008-0
10.1016/j.soilbio.2013.08.017
https://www.citeqbiologics.com/difference-between-bca-bradford-protein-assays/
https://bitesizebio.com/23824/top-5-protein-quantification-assays/
The Bradford assay is one of the most commonly used methods for protein quantification.However ,short-chain alcohols and citric and ascorbic acids can be used instead of the ethanol and phosphoric acid.
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